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Journal of Spectroscopy
Volume 2019, Article ID 1521035, 8 pages
Research Article

An Approach to Rapid Determination of Tween-80 for the Quality Control of Traditional Chinese Medicine Injection by Partial Least Squares Regression in Near-Infrared Spectral Modeling

1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
2Nansha Research Institute, Sun Yat-sen University, Guangzhou 511458, China
3Guangdong Pharmaceutical University, Guangzhou 510006, China

Correspondence should be addressed to Xue Xiao; moc.361@yppahoaixre and Fa-Huan Ge; nc.ude.usys.liam@nauhafeg

Received 18 May 2019; Accepted 22 July 2019; Published 22 August 2019

Academic Editor: Jose S. Camara

Copyright © 2019 Jin-Fang Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study established an approach to rapidly determine the Tween-80 in traditional Chinese medicine (TCM) injection by using near-infrared (NIR) spectroscopy. Totally 133 standard solutions of Tween-80 were prepared and randomly divided into calibration set and validation set, containing 109 and 24 samples, respectively. Spectral data were preprocessed and then subjected to establish a predictive model using partial least-squares (PLS). The standard error of cross validation (SECV), standard deviation of calibration (SEC), and the determination coefficient (R) of the established model were 0.0561, 0.0526, and 0.9986, respectively. The model was successfully applied to determine Tween-80 contents in 25 XBJ Injections and 40 FFSX Injections, and it produced satisfactory quantitative analysis results with average relative deviations 0.60% and 0.16%, respectively, for 25 XBJ Injections and 40 FFSX Injections, and the maximum relative deviations 8.57% and 7.60%, respectively. This work shows that NIR model displayed quite good predictive ability for Tween-80 quantitative analysis, which could potentially be applied to rapid determination of Tween-80 in the production process of TCM injections and other TCM products.