Chemometrics-Enabled Raman Spectrometric Qualitative Determination and Assessment of Biochemical Alterations during Early Prostate Cancer Proliferation in Model Tissue

Figure 3

Difference spectra between the normalized mean spectra of PC3 and PNT1a cells for (a) stage 1 (48 hours), (b) stage 2 (72 hours), and (c) stage 3 (96 hours) spectral measurements. It is observed the PC3 samples have prominent spectral markers at 566 ± 0.70 cm⁻¹, 630 cm⁻¹, 972 ± 1.17 cm⁻¹, 1186 cm⁻¹, 1520 ± 1.41 cm⁻¹, and 1743 cm⁻¹. The PNT1a samples have prominent spectral markers at 550 ± 0.23 cm⁻¹, 719 ± 1.31 cm⁻¹, 852 ± 0.47 cm⁻¹, 948 ± 1.88 cm⁻¹ 1250 ± 2.86 cm⁻¹, 1332 ± 1.64 cm⁻¹, 1450 ± 2.20 cm⁻¹, and 1660 cm⁻¹.

(a)

(b)

(c)