Investigation on the Binding and Conformational Change of All-trans-Retinoic Acid with Peptidyl Prolyl cis/trans Isomerase Pin1 Using Spectroscopic and Computational Techniques

Figure 5

ATRA affects the conformation of Pin1. (a) CD spectra of Pin1 in the absence and presence of ATRA. c_Pin1 = 10 μM; c_ATRA = 0 (black line), 1 (red line), and 3 (blue line) μM. (b, c) Synchronous fluorescence spectra of Pin1 in the absence and presence of ATRA with Δλ = 15 nm and 60 nm, respectively. c_Pin1 = 5 μM; c_ATRA = 0 (black line), 0.5 (red line), 1 (blue line), 2 (magenta line), 3 (olive line), 4 (navy line), and 5 (violet line) μM. (d, e) Three-dimensional fluorescence spectra of Pin1 in the absence and presence of ATRA; c_Pin1 = 5 μM and c_ATRA = 0 and 5 μM.

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