Mathematically Processed UV Spectroscopic Method for Quantification of Chlorthalidone and Azelnidipine in Bulk and Formulation: Evaluation of Greenness and Whiteness

Table 1

Validation parameter results of the proposed spectroscopic methods for the simultaneous determination of CTL and AZE.

Validation parameters	Chlorthalidone				Azelnidipine
	^1DD	RAD	R^1DD	RCS	^1DD	RAD	R^1DD	RCS
Wavelength (nm)	238.5	211.8–267.2	239.8	226.9	272.1	328.4–286.1	342.1	257.3
Linearity range (μg/mL)	1–15	1–15	1–15	1–15	1–15	1–15	1–15	1–15
Slope	0.3025	1.0924	0.0367	0.0684	0.1129	16.441	0.4986	0.0432
Intercept	−0.0772	−0.3106	−0.0091	−0.0063	−0.0129	8.8035	0.1193	−0.0089
Regression coefficient (r^2)	0.9996	0.9998	0.9998	0.9997	0.9999	0.9997	0.9997	0.9995
LOD (μg/mL)	0.305	0.267	0.408	0.306	0.128	0.301	0.186	0.328
LOQ (μg/mL)	0.924	0.768	0.806	0.928	0.387	0.933	0.599	0.995
Accuracy (mean % ± RSE)	100.24 ± 0.92	98.95 ± 1.04	99.47 ± 1.34	99.27 ± 1.61	98.37 ± 1.48	99.46 ± 0.54	100.34 ± 1.49	100.38 ± 1.41
Precision (%RSD)
Intraday	1.053	0.945	1.438	0.893	1.232	0.715	1.758	1.422
Interday	1.127	0.846	0.807	1.803	1.459	0.397	0.931	1.087

RSE: Relative standard error; RSD: Relative standard deviation.