Mathematically Processed UV Spectroscopic Method for Quantification of Chlorthalidone and Azelnidipine in Bulk and Formulation: Evaluation of Greenness and Whiteness

Table 3

Assay results of the formulation and the standard addition method results.

Formulation concentration		Chlorthalidone (mean % ± SD)				Azelnidipine (mean % ± SD)
CTL	AZE	^1DD	RAD	R^1DD	RCS	^1DD	RAD	R^1DD	RCS
6.25 mg	8 mg	100.28 ± 0.54	98.79 ± 1.04	100.49 ± 0.75	99.39 ± 1.34	100.92 ± 0.75	99.67 ± 1.71	100.32 ± 0.62	99.44 ± 0.67
12.5 mg	8 mg	99.47 ± 1.42	98.76 ± 0.94	99.76 ± 1.81	100.43 ± 0.88	99.53 ± 1.66	98.85 ± 0.98	100.54 ± 0.84	101.24 ± 1.78
Standard addition method
Amount added (μg/mL)		Chlorthalidone (% recovery)				Azelnidipine (% recovery)
3.125	2	100.49	99.75	100.64	101.39	100.34	99.37	100.08	100.49
6.25	4	99.78	101.66	99.45	99.34	99.42	98.55	99.36	99.78
9.375	6	100.24	99.46	98.76	98.71	101.84	100.42	98.7	100.24
Across mean		98.71	100.17	100.29	99.62	99.81	100.53	99.45	99.38
%RSD		0.36	1.19	0.95	1.40	1.22	0.94	0.69	0.36

SD: standard deviation.