Journal of Signal Transduction / 2012 / Article / Fig 3

Review Article

Nuclear Transport: A Switch for the Oxidative Stress—Signaling Circuit?

Figure 3

Oxidative stress interferes with importin-α1-dependent import of endogenous cargos. The import of two endogenous proteins, the RNA-binding protein HuR and galectin-3, was monitored in HeLa cells under the conditions described for Figure 2(a). Importin-α1 promotes nuclear import of both proteins. HuR and galectin-3 were visualized by indirect immunofluorescence and nuclei were stained with DAPI [69]. HuR was nuclear in control cells and redistributed to the cytoplasm of stressed cells, where it accumulated in stress granules (SGs). Similarly, galectin-3 was more concentrated in the nuclei of control cells and relocated to the cytoplasm upon DEM treatment. Arrows indicate the position of some of the SGs.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.