Journal of Toxicology / 2009 / Article / Fig 1

Research Article

Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways

Figure 1

Biz-2 modulates PSA secretion by LNCaP cells. LNCaP cells (1E7) were grown in serum-free medium for 24 hours, then induced with 100 ppm of Biz-2 for 0, 6, 12, and 24 hours. Equal aliquots (10 uL) of the condition medium were removed and analyzed by PSA ELISA (see Section 2). (a) Time-dependent secretion of PSA. (b) PSA secretion induced with 10 ppm Biz-2 for 24 hours in the presence and absence of 10 nM flutamide. The values are the mean ± SEM of three separate experiments performed in triplicate. CON: control; DHT: dihydrotestosterone; Biz-2: ether extract of Bizzy nut; RES: resveratrol.