Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways
Figure 1
Biz-2
modulates PSA secretion by LNCaP cells. LNCaP cells (1E7) were grown in serum-free medium for 24 hours,
then induced with 100 ppm of Biz-2 for 0, 6, 12, and 24 hours. Equal aliquots
(10 uL) of the condition medium were removed and analyzed by PSA ELISA (see Section 2). (a) Time-dependent
secretion of PSA. (b) PSA secretion
induced with 10 ppm Biz-2 for 24 hours in the presence and absence of 10 nM
flutamide. The values are the mean ± SEM of three
separate experiments performed in triplicate. CON: control; DHT:
dihydrotestosterone; Biz-2: ether extract of Bizzy nut; RES: resveratrol.
