Journal of Toxicology / 2009 / Article / Fig 5

Research Article

Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways

Figure 5

Modulation of prostate cell proliferation by Bizzy nut extracts. LNCaP or DU145 cells were grown in the presence of 0–1000 ppm Biz-2 for 24 hours, and cell growth was determined using (a) 3[H] thymidine incorporation, (b) MTT cell viability assay and tryphan blue dye staining, and (c) cytotoxicity of Biz-2 in LNCaP cells that was performed as described in Section 2. Growth inhibitory activity of the extracts was determined as . Each value represents mean ± SEM for three experiments performed in eight wells of a 96-well plate. Values followed by differed significantly from the controls.