Journal of Toxicology / 2009 / Article / Fig 6

Research Article

Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways

Figure 6

Cellular and nuclear morphology indicative of apoptosis induced by Biz-2. LNCaP cells were grown on a microscope slide, then induced with 1 or 100 ppm of Biz-2 for 24 hours. Cells were stained with a solution of acridine orange and ethidium bromide for 5 minutes, then examined by light microscopy. (a) Confocal laser fluorescent microscope images taken with a 550 nm filter (Top panel: acridine orange, middle panel: ethidium bromide, bottom panel: both). (b) Apoptotic index was determined. We calculate the apoptotic index by averaging the number of apoptotic cells per field (70–60 cells), then dividing by the total number of cells perfield (120–140 cells) (total number of cells − total number of apoptotic cells)/(total number of cells).