Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways
Figure 6
Cellular and nuclear morphology indicative of
apoptosis induced by Biz-2. LNCaP cells were grown on a microscope slide, then
induced with 1 or 100 ppm of Biz-2 for 24 hours. Cells were stained with a
solution of acridine orange and ethidium bromide for 5 minutes, then examined
by light microscopy. (a) Confocal
laser fluorescent microscope images taken with a 550 nm filter (Top panel: acridine
orange, middle panel: ethidium bromide, bottom panel: both). (b) Apoptotic index was determined. We calculate the apoptotic index by
averaging the number of apoptotic cells per field (70–60 cells), then
dividing by the total number of cells perfield (120–140 cells) (total number of cells − total number of
apoptotic cells)/(total number of cells).
