Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways
Figure 8
Biz-2 increases
Caspase 3/7 levels in LNCaP cells. LNCaP
cells (1 × 106) were grown in test medium and induced with 100 ppm
Biz-2 for 24 hours, and the resulting Caspase 3/7 activity was determined using
the Apo-One Homogeneous Caspase-3/7 assay as described in Section 2. (a) Western blot of Bcl2 and Bax proteins following
induction of LNCaP with Biz-2 in the absence and presence of flutamide. (b) Caspase 3/7 activity was expressed as
n-fold increase relative to untreated control (n = 8, mean ± SEM).
