Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells

Figure 2

Morphological changes in SCC25 and HaCaT cells after the genus Sinularia extracts treatment. A constant concentration of extracts (IC₅₀) was added to the cells for 18 hours. The change was inspected by a phase-contrast-inverted microscopy. The cells were then fixed in 4% paraformaldehyde and DNA stained with Hoechst. The nuclei of the cells were visualized using a fluorescent microscope (200×). S. grandilobata, 1; S. parva, 2; S. triangula, 3; S. scabra, 4; S. nanolobata, 5; and S. gibberosa, 6.