Journal of Toxicology

Research Article

Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells

Table 3

The changes of cell cycle distribution of the genus Sinularia extracts in SCC25 and HaCaT cells.


Cell lines	No.	Treatment (μg/mL)	Sub-G₁ phase		G₀/G₁ phase		S phase		G₂/M phase
Cell lines	No.	Treatment (μg/mL)	Mean	%	Mean	%	Mean	%	Mean	%

SCC25	Control	0	1.0	100.0	52.3	100.0	19.8	100.0	27.2	100.0
	1	IC₅₀	32.5	3250.0	37.7	72.1	16.5	83.3	13.8	50.7
	1	IC₈₀	64.1	6410.0	21.9	41.9	7.3	36.9	6.8	25.0
	2	IC₅₀	18.7	1870.0	46.8	89.4	15.7	79.3	19.8	72.8
	2	IC⁸⁰	58.9	5890.0	24.1	46.1	8.3	41.9	8.8	32.4
	3	IC₅₀	23.6	2360.0	54.3	103.8	12.2	61.6	10.5	38.6
	3	IC⁸⁰	69.8	6980.0	18.6	35.6	6.8	34.4	4.8	17.6
	4	IC₅₀	33.4	3340.0	37.7	72.1	14.4	72.7	14.8	54.4
	4	IC₈₀	80.1	8010.0	13.4	25.6	3.6	18.2	3.1	11.4
	5	IC⁵⁰	24.3	2430.0	51.1	97.7	12.5	63.1	12.5	46.0
	5	IC₈₀	36.8	3680.0	40.4	77.2	12.8	64.6	10.3	37.9
	6	IC⁵⁰	33.7	3370.0	32.8	62.7	15.5	78.3	18.3	67.3
	6	IC₈₀	79.6	7960.0	12.4	23.7	4.4	22.2	3.6	13.2

HaCaT	Control	0	0.8	100.0	59.5	100.0	16.2	100	23.7	100.0
	1	IC⁵⁰	35.3	4412.5	34.3	57.6	13.1	80.9	16.7	70.5
	1	IC₈₀	69.4	8675.0	6.7	11.3	7.9	48.8	16.1	67.9
	2	IC₅₀	19.4	2425.0	42.8	71.9	17.8	109.9	20.3	85.7
	2	IC₈₀	52.3	6537.5	15.3	25.7	15.1	93.2	17.4	73.4
	3	IC₅₀	25.1	3137.5	45.2	76.0	16.4	101.2	13.9	58.6
	3	IC⁸⁰	56.1	7012.5	13.2	22.2	16.1	100	15.1	63.7
	4	IC⁵⁰	39.1	4887.5	38.2	64.2	13.1	80.9	9.6	40.5
	4	IC₈₀	71.3	8912.5	8.1	13.6	9.9	61.1	10.9	46.0
	5	IC₅₀	35.8	4475.0	35.4	59.5	15.4	95.1	13.8	58.2
	5	IC₈₀	58.9	7362.5	11.9	20.0	14.3	88.3	15.1	63.7
	6	IC⁵⁰	57.4	7175.0	10.2	17.1	12.8	79.0	19.7	83.1
	6	IC₈₀	72.9	9112.5	5.5	9.2	8.3	51.2	13.3	56.1

(i) S. grandilobata, 1; S. parva, 2; S. triangula, 3; S. scabra, 4; S. nanolobata, 5; S. gibberosa, 6.