In Vitro Study of Mutagenesis Induced by Crocidolite-Exposed Alveolar Macrophages NR8383 in Cocultured Big Blue Rat2 Embryonic Fibroblasts

Figure 2

ROS production in NR8383 analysed by flow cytometry. NR8383 cells were preincubated with H₂DCFDA (25 $\mu$m) for 30 minutes and then exposed to 10 $\mu$g/cm² of crocidolite or zymosan for 3 hours. Representative histograms plot the relative green DCF fluorescence (FL1) within 15000 live cells (counts: numbers of events).