Research Article
In Vitro Study of Mutagenesis Induced by Crocidolite-Exposed Alveolar Macrophages NR8383 in Cocultured Big Blue Rat2 Embryonic Fibroblasts
Table 3
Cytotoxicity and mutation frequencies in Big Blue cells following various treatments in mono- or coculture with NR8383 cells.
| Treatment and culture condition | | % cell mortality (Mean SD) | PFU per assay | Mutant plaques | MF (10−5) | Group average MF (10−5 SD) |
| ENU, monoculture, 30 min | Control | 1.2 0.5 | 375000 | 32 | 8.5 | 9.6 1.0 | | | | 350000 | 36 | 10.3 | | | | | 455000 | 46 | 10.1 | |
| | 100 g/mL | 2.3 1.4 | 285000 | 133 | 46.7 | 41.8 8.7* | | | | 553333 | 176 | 31.8 | | | | | 338330 | 159 | 47.0 | |
| | 500 g/mL | 2.2 0.5 | 223000 | 208 | 93.3 | 83.3 9.8* | | | | 368330 | 306 | 83.1 | | | | | 203666 | 150 | 73.6 | | | | | | | | |
| Crocidolite separated coculture, 72 h | Control | 4.1 1.9 (BB); 5.4 2.3 (NR) | 423334 | 47 | 11.1 | 12.9 1.6 | | | | 860333 | 102 | 11.9 | | | | | 868333 | 122 | 14.0 | | | | | 566667 | 79 | 13.9 | | | | | 675000 | 79 | 11.7 | | | | | 573333 | 86 | 15.0 | |
| | 3 g/cm2 | 5.8 2.0 (BB); 7.9 2.0 (NR) | 349000 | 25 | 7.2 | 8.7 1.7 | | | | 477333 | 41 | 8.6 | | | | | 506166 | 53 | 10.5 | |
| | 15 g/cm2 | 3.6 1.8 (BB); 19.6 1.3* (NR) | 1090000 | 127 | 11.7 | 13.2 2.2 | | | | 786667 | 77 | 9.8 | | | | | 445000 | 70 | 15.7 | | | | | 723333 | 104 | 14.4 | | | | | 567667 | 82 | 14.4 | | | | | 616667 | 81 | 13.1 | |
| Crocidolite, mixed coculture, 24 h | Control | 8.2 0.9 | 303350 | 63 | 20.8 | 19.0 2.3 | | | | 303350 | 57 | 18.8 | | | | | 301650 | 68 | 22.5 | | | | | 460000 | 79 | 17.2 | | | | | 501650 | 83 | 16.5 | | | | | 388350 | 71 | 18.3 | |
| | 10 g/cm2 | 15.0 1.4* | 246650 | 43 | 17.4 | 18.5 4.4 | | | | 246650 | 63 | 25.5 | | | | | 331650 | 71 | 21.4 | | | | | 475333 | 61 | 12.8 | | | | | 692333 | 122 | 17.6 | | | | | 323350 | 52 | 16.1 | |
| Zymosan, mixed coculture, 3 h | Control | 7.1 2.0 | 468333 | 69 | 14.7 | 13.0 1.5 | | | | 248000 | 30 | 12.1 | | | | | 593333 | 72 | 12.1 | |
| | 15 g/cm2 | 6.4 0.8 | 525000 | 82 | 15.6 | 18.3 2.9* | | | | 656667 | 118 | 18.0 | | | | | 393334 | 84 | 21.4 | |
| | 30 g/cm2 | 7.0 0.9 | 471667 | 83 | 17.6 | 17.4 1.4* | | | | 833334 | 156 | 18.7 | | | | | 600000 | 96 | 16.0 | |
| | 60 g/cm2 | 7.7 1.8 | 465000 | 134 | 28.8 | 30.8 7.0* | | | | 574167 | 144 | 25.1 | | | | | 513333 | 198 | 38.6 | |
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PFU: plaque forming unit; MF: mutant frequency; BB: Big Blue; NN: NR8383; *Statistically different from control values ().
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