Shortening and Improving the Embryonic Stem Cell Test through the Use of Gene Biomarkers of Differentiation
Figure 1
Effect of 5-FU, DPH, and saccharin on cell viability. D3 and 3T3 cells were cultured on monolayers exposed to 5-FU (a), DPH (b), or saccharin (c) for 5 days. Then the MTT test was performed as described in Section 2. D3 cells were cultured under spontaneous differentiation (in the absence of LIF). The results are expressed as a percentage of absorbance at 570 nm with regards to the control cultures, which were not exposed to 5-FU, DPH, or saccharin. Each condition was assayed with twelve independent wells.
