The Loss of HIF1 Leads to Increased Susceptibility to Cadmium-Chloride-Induced Toxicity in Mouse Embryonic Fibroblasts
Figure 1
Characterization of cadmium-induced cell death. (a) Wild-type (WT, white bars) and HIF1α −/− (black bars) cells were left untreated (0) or exposed to 1, 2, 5, 10 μM CdCl2 for 72 hours. Cell viability was assessed using a standard MTT assay. Untreated control values within cell type were set to 1. * compared to the corresponding controls, . (b) Wild-Type (WT, solid line) and HIF1α −/− (Null, dashed line) were left unexposed (Control, circles) or Cadmium (Cd, 5 μM, triangles) for various times and cell viability was measured using MTT assay. * compared to the corresponding controls. (c) Wild-type (WT) and HIF1α −/− cells were left untreated (Control) or exposed to 5 μM CdCl2 for 24 hours and nuclear morphology was observed after staining with Hoechst 33342 dye using fluorescence microscopy. (d) Wild-type (WT, white bars) and HIF1α −/− (black bars) cells were left untreated (Ctrl) or exposed to CdCl2 (5 μM, 24 hours), or staurosporine (1 μM, 4 hours). Caspase-3 activity was measured using EnZChek caspase-3 assay kit number 2 (Molecular Probes). * compared to the corresponding controls, . #significant compared to wild type, , .
