The Loss of HIF1 Leads to Increased Susceptibility to Cadmium-Chloride-Induced Toxicity in Mouse Embryonic Fibroblasts
Figure 3
Oxidative Stress in CdCl2-mediated cytotoxicity. (a) Wild-type (WT) and HIF1α −/− cells were left untreated (Ctrl), or exposed to 2 mM H2O2 (H2O2, 2 hours), or 10 μM CdCl2 (Cd2+, 24 hours). Reactive oxygen species generated in the cell were measured using the ROS-sensitive dye, CH-H2DCFDA, and flow cytometry. Arrow points to the median fluorescence intensity of control cells. (b) Wild-type (white bars) and HIF1α −/− (black bars) cells were treated with 10 μM CdCl2 (Cd2+) alone or with 10 mM N-acetyl cysteine (NAC) for 24 hours and cell viability was measured using MTT assay. (c)–(f) Wild-type (white bars) and HIF1α −/− (black bars) cells were treated with 5 μM CdCl2 (Cd2+) alone or with 1 mM reduced glutathione (GSH, C), 1 mM oxidized glutathione (GSSG, D), 0.5 mM Melatonin (MEL, E), 0.2% ethanol (ETH, E) or 0.5 mM ascorbic acid (Asc., F) and cell viability was measured using MTT assay. Control values within cell type were set to 1. *significant compared to cadmium treatment within each cell type, , .
