The Loss of HIF1 Leads to Increased Susceptibility to Cadmium-Chloride-Induced Toxicity in Mouse Embryonic Fibroblasts
Figure 4
Cadmium-induced changes in metallothionein and MTF-1. Wild-type (WT, white bars) and HIF1α −/− (black bars) cells were left untreated (Ctrl) or 5 μM CdCl2 (Cd2+) for 24 hours. Metallothionein-1 (A, MT-1), Metallothionein-2 (B, MT-2), and Metal Transcription Factor-1 (C, MTF-1) mRNA levels were measured using qRT-PCR. All values were normalized to wild-type control. The number above each bar is the fold change for that particular treatment. * compared to respective controls, # compared to wild-type cells within treatment group, . G. Metallothionein-1/2 (middle panel) and MTF-1 (lower panel) protein levels were determined in wild-type and HIF1α −/− cells after treatment with 5 μM CdCl2 (Cd2+) for 24 hours. β-actin was used as a loading control (upper panel).
