Sulfurous Gases As Biological Messengers and Toxins: Comparative Genetics of Their Metabolism in Model Organisms

Figure 2

Phylogenetic tree analysis of cystathionine-β-synthase (CBS) and putative cysteine synthase (PCS). A blast (blastp) search of known Homo sapiens (H.s) proteins was undertaken against three different species, Saccharomyces cerevisiae (S.c), Drosophila melanogaster (D.m), and Caenorhabditis elegans (C.e) in the Genbank database. Identified sequences with significant expected value (≤1E−10) were used to generate a multiple sequence alignment (MSA) via ClustalW 2.1. The MSAs were then trimmed and used to produce an unrooted phylogenetic tree with 10,000 boostraps via Geneious 5.4.