Research Strategies in the Study of the Pro-Oxidant Nature of Polyphenol Nutraceuticals
Comparative generation of hydrogen peroxide (H2O2) in phosphate buffer, maintained at different pH levels, and in cell culture medium supplemented with Gingko biloba extract. The Dulbecco’s modified Eagle medium (DMEM) in this study was amended 10% Serum Plus, 2% fetal bovine serum, and antimicrobial agents and was the medium in which the cells were exposed to the test agents. H2O2, generated by the plant extract, was quantified with the FOX assay, after a 2-hour incubation at room temperature. Data, from Babich et al. , are presented as the mean percent of H2O2 ± S.E.M.