Domoic Acid-Induced Neurotoxicity Is Mainly Mediated by the AMPA/KA Receptor: Comparison between Immature and Mature Primary Cultures of Neurons and Glial Cells from Rat Cerebellum
Figure 3
Changes in the mRNA levels of astrocytic and neural precursor markers in primary cultures of CGCs exposed to domoic acid (5 μM, 10 μM, and 20 μM) at 1 DIV (immature cultures) or at 7 DIV (mature cultures) for 3 or 10 days. There were no observed changes for (a) GFAP in immature cultures, (c) S100β in immature cultures, and (e) nestin expression in immature cultures. However, there was a significant decrease of (b) GFAP mRNA levels in mature cultures after 10 days of exposure (20 μM) and increase of (d) S100β mRNA expression in mature cultures after 10 days of exposure and (f) nestin mRNA expression in mature cultures after 10 days of exposure. Gene expression levels were normalized to the standard calibrator, the housekeeping gene 18S rRNA and the mRNA expression at 1 DIV. Data represent mean ± S.E.M. of three independent experiments performed in duplicates. ++++++ comparing control cultures at 3 and 10 days. * ** *** comparing treated to control (untreated) cultures.
