Domoic Acid-Induced Neurotoxicity Is Mainly Mediated by the AMPA/KA Receptor: Comparison between Immature and Mature Primary Cultures of Neurons and Glial Cells from Rat Cerebellum
Figure 6
Changes in the mRNA levels of astocytic and precursor markers in mature primary cultures of CGCs exposed to 20 μM domoic acid alone or in combination with antagonists for the AMPA-R (NBQX, 20 μM), NMDA-R (APV, 100 μM) or the neurotransmitter GABA (10 μM) at 7 DIV for 3 or 10 days. NBQX but not APV or GABA prevented the domoic acid induced downregulation of (a) GFAP mRNA expression. Note that NBQX, APV and GABA could prevent the domoic acid induced up-regulation of (b) S100β and (c) nestin mRNA levels. Gene expression levels were normalized to the standard calibrator, the housekeeping gene 18S rRNA and the mRNA expression at 1 DIV. Data represent mean ± S.E.M. of three independent experiments performed in duplicates. * ** *** comparing treated to control (un-treated) cultures.
