Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease
Figure 4
Western blotting reveals a disconnect between the UPR and oxidative stress in the pathogenesis of ALD. (a) Standard western blotting shows a lack of activation in classical UPR signaling cascades at any time point. (b) No induction of the Ero1 proteins or Nox4 was observed, confirming a lack of UPR-induced oxidative stress. β-actin was utilized as a loading control for total liver extracts while Lamin B1 was used as a nuclear loading control.
