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Journal of Toxicology
Volume 2012 (2012), Article ID 293784, 9 pages
Research Article

In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)

1Interdisciplinary Nanoscience Center (iNANO) and Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C, Denmark
2Section of Zoophysiology, Department of Biological Sciences, Aarhus University, C.F. Moellers Allé 3, Building 1131, 8000 Aarhus C, Denmark

Received 31 May 2011; Accepted 8 September 2011

Academic Editor: Christian Gagnon

Copyright © 2012 Katrine Bilberg et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table 1: Silver nanoparticle size by Dynamic Light Scattering (DLS). The used silver nanoparticles were coated with 0.2% polyvinyl pyrrolidone (PVP) (stated by the manufacturer to be spherical 30–40 nm particles). Particle suspensions were subjected to sodium chloride contractions (100 mg L−1 to 800 mg L−1) at stock pH value 3.9 and at the head tank (fish tank water) equivalent pH value 6.9, followed over 24 hours at 26°C. The samples were kept between measurements under conditions identical to the fish tanks used for nanosilver toxicity assessment. DLS measurement was run in triplicate using automated, optimal measurement time and laser attenuation settings.

  1. Supplementary Material