Thimerosal-Derived Ethylmercury Is a Mitochondrial Toxin in Human Astrocytes: Possible Role of Fenton Chemistry in the Oxidation and Breakage of mtDNA

Figure 6

Summary of observed changes in NHA following incubation with ethylmercury. Bar plot showing the summarized changes observed in NHA following a one-hour exposure to 14.4 𝜇M Thimerosal, with respect to untreated controls. Each value is expressed as mean ± SD of five fields measured in the center field of triplicate experiments. Each of the treated values is statistically different from the controls at 𝑃<0.01. The increase in HO^• is statistically different from the increase in H₂O₂ (H₂DCF-AM) at 𝑃<0.01. Statistical analyses were performed using one-way analysis of variance (ANOVA) with the Holm-Bonferroni post hoc test. The test was performed only when the results of ANOVA were 𝑃<0.05, using Daniel’s XL Toolbox, a free, open source add-in for Microsoft Excel.