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Journal of Toxicology
Volume 2012, Article ID 951568, 6 pages
Research Article

Integrated Use of Biomarkers (O : N Ratio and Acetylcholinesterase Inhibition) on Aulacomya ater (Molina, 1782) (Bivalvia: Mytilidae) as a Criteria for Effects of Organophosphate Pesticide Exposition

1Centro Regional de Estudios Ambientales (CREA), Universidad Católica de la Santísima Concepción, P.O. Box 297, Concepción, Chile
2Departamento de Química Ambiental, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Concepción 4090541, Chile

Received 28 September 2011; Revised 29 January 2012; Accepted 3 February 2012

Academic Editor: Darakhshan Jabeen Haleem

Copyright © 2012 Eduardo Führer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effect of residual concentrations of organophosphate pesticide chlorpyrifos (Lorsban 4E) on the activity of the acetylcholinesterase enzyme and oxygen : nitrogen ratio in the mussel Aulacomya ater was analyzed. Toxicity tests show a sensitivity to the pesticide in the bivalve estimated at 16 μg L-1 (LC50-96 hours). Concentrations between 0.2 and 1.61 μg L-1 were able to inhibit significantly the AChE activity, and concentrations between 0.8 and 1.61 μg L-1 stimulate ammonia excretion and decrease oxygen : ammonia-N (O : N) ratio, with respect to the control group. A. ater proved to be a species sensitive to pesticide exposure and easy to handle in lab conditions. Thus, it is recommended as a bioindicator for use in programs of environmental alertness in the Eastern South Pacific coastal zone.