Specific Conditions for Resveratrol Neuroprotection against Ethanol-Induced Toxicity
Figure 4
Histograms of the distribution of comet tail DNA obtained after a 3 day exposure of astrocytes to 20 mM ethanol, preceded or not by a 1 h pretreatment with resveratrol (5 μM) and followed or not by a 1 h or 3 h recovery period in fresh culture medium. (a) Control; (b) pretreatment with resveratrol; (c) 3-day exposure to ethanol; (d) pretreatment with resveratrol followed by exposure to ethanol; 1 h recovery (e) and 3 h recovery (g) of astrocytes after ethanol exposure; 1-h recovery (f) and 3 h recovery (h) of astrocytes after ethanol exposure when cells were first pretreated with resveratrol. (a) versus (c), (d) versus (c), (f) versus (e), , Kolmogorov-Smirnov test. Experimental conditions are detailed in Section 2. Insets show representative micrographs of “comet,” corresponding to the various cell treatments described in the legend.
