Journal of Toxicology

Research Article

Alterations in Sensitivity to Estrogen, Dihydrotestosterone, and Xenogens in B-Lymphocytes from Children with Autism Spectrum Disorder and Their Unaffected Twins/Siblings

Table 3

Comparison of changes in XTT/LDH ratio.


Drug	Abs	Ord		Measured slope 90% CI	Critical slope 90% CI	P < 0.01

E2	AUT	Bro	0.952	1.579–1.1	1.443–0.327	—
	AUT	Sis	0.970	1.722–1.303	1.431–0.318	—
	AUT	CON	0.996	2.245–2.032	1.402–0.296	**
	Bro	Sis	0.995	1.181–1.05	1.569–0.359	—
	Bro	Con	0.971	1.748–1.327	1.537–0.339	—
	Sis	Con	0.987	1.514–1.257	1.516–0.333	—

DHT	AUT	Bro	0.848	1.291–0.641	1.504–0.358	—
	AUT	Sis	0.975	1.611–1.249	1.372–0.264	—
	AUT	CON	0.997	2.144–1.959	1.556–0.395	**
	Bro	Sis	0.941	1.604–1.074	1.519–0.311	—
	Bro	Con	0.874	2.382–1.28	1.723–0.434	—
	Sis	Con	0.985	1.547–1.269	1.502–0.379	—

DDE	AUT	Bro	0.942	1.522–1.021	1.432–0.311	—
	AUT	Sis	0.961	1.677–1.213	1.438–0.316	—
	AUT	CON	0.993	2.215–1.936	1.478–0.344	**
	Bro	Sis	0.996	1.182–1.064	1.504–0.337	—
	Bro	Con	0.973	1.775–1.361	1.545–0.364	—
	Sis	Con	0.985	1.538–1.267	1.556–0.367	—

Table 3 shows comparison of the changes in XTT/LDH ratio of the four cell types and three effectors and is based on Figure 2 cumulative sum plots. All fitted plots produced a linear trend where the rho value of the fit was <0.01. Columns 1–3 of Table 2 show effector examined and the pair of cell types whose correlation is compared. The statistic of the resultant slope is shown in the 4th column. The 5th column shows the 90% confidence interval of the generated slope. The 6th column indicates the theoretical 90% confidence interval around a slope of 1, calculated from the variance of each pair of cell type’s response to the tested effector. The final (7th) column indicates if the growth response of the two cell populations in question differs at the P = 0.01 confidence level. AUT and Con were the only 2 groups that showed a significantly different response to the 3 effectors (E2, DHT, and DDE).
ASD: autism spectrum disorder; AUT: B cells from individual with ASD; Bro: B cells from brother of individual with ASD; CI: confidence interval; Con: control B cells from individual with no personal or family history of ASD; DDE: dichlorodiphenyldichloroethylene; DHT: dihydrotestosterone; E2: estradiol; LDH: lactate dehydrogenase; Sis: B cells from phenotypically normal sister of individual with ASD; XTT: 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide; XTT/LDH: ratio of XTT/LDH indicative of mitochondrial function per cell.