Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn

Figure 4

t-Butylhydroperoxide induced chemiluminiscence in rat uterine horn homogenates from rats receiving an alcohol containing liquid diet. Rat uterine horn tissue homogenized in 0.25 M sucrose, 50 M deferoxamine in TKM buffer, pH 7.5 (≈7 mg protein/mL) was kept at 37°C for 10 min in a Dubnoff shaker. Chemiluminiscence measurement was started by addition of 3 mM t-butylhydroperoxide. Values are the mean ± S.D. Three samples per group were run, each consisting of a homogenate from a separate lot of pooled uterine horn tissue (five animals each).