Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn

Table 2

Ethanol metabolism to acetaldehyde in the microsomal fraction of uterine horn tissue from rats receiving an alcohol containing liquid diet.

Experimental^a	Acetaldehyde (nmol/mg protein)
	Control	EtOH-treated^b
−NADPH	1.67±0.10	1.86±0.10
+NADPH^c	2.11±0.07	2.67±0.40

aIncubation mixtures containing microsomal fraction (0.17–0.19 mg protein/mL), 0.14 M ethanol, and, when indicated, NADPH generating system (0.45 mM NADP⁺, 4 mM d,l-isocitric acid trisodium salt, and 0.25 units of isocitric dehydrogenase) in KH₂PO₄ buffer were conducted for 1 hour at 37°C. Acetaldehyde was measured in the head space of each sample after adding 1 mL NaCl saturated solution. See Section 2 for details. Each result is the mean of three separate lots of pooled uterine tissue samples.
^b𝑃<0.05 when compared to Control versus EtOH-treated.
^c𝑃<0.05 when compared to −NADPH versus +NADPH.