Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn

Table 3

Ethanol metabolism to acetaldehyde in the cytosolic fraction of uterine horn tissue from rats receiving an alcohol containing liquid diet.

Experimental^a	Acetaldehyde (nmol/mg protein)
	Control	EtOH-treated
Ethanol only	1.39±0.03	1.78±0.05
Ethanol + NAD	1.67±0.04^b	0.43±0.03^g
Ethanol + NAD + HX	1.76±0.13^c,e	0.32±0.01^g,h
Ethanol + NAD + HX + allopurinol	0.13±0.01^c,d,f	0.27±0.06^g,i,j

aIncubation mixtures containing cytosolic fraction (1.4–1.6 mg protein/mL), 0.14 M ethanol, and, when indicated, 0.25 mM hypoxanthine (HX) and 0.3 mM NAD⁺ in STKM buffer were conducted for 1 h at 37°C. Acetaldehyde was measured in the head space of each sample after adding 1 mL NaCl saturated solution. See Section 2 for details. Each result is the mean of three separate lots of pooled uterine tissue samples.
^b𝑃<0.01 when compared to “Ethanol only” (Control).
^c𝑃<0.001 when compared to “Ethanol only” (Control).
^d𝑃<0.001 when compared to “Ethanol + NAD + HX” (Control).
^e𝑃>0.05 when compared to “Ethanol + NAD” (Control).
^f𝑃<0.001 when compared to “Ethanol + NAD” (Control).
^g𝑃<0.001 when compared to “Ethanol only” (EtOH-treated).
^h𝑃<0.05 when compared to “Ethanol + NAD” (EtOH-treated).
ⁱ𝑃>0.05 when compared to “Ethanol + NAD + HX” (EtOH-treated).
^j𝑃<0.01 when compared to “Ethanol + NAD” (EtOH-treated).