Research Article
Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn
Table 4
Protein sulfhydryl and protein carbonyl in uterine horn from rats receiving an alcohol containing liquid diet.
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Uterine horn tissue isolated from Control and EtOH-treated rats was homogenized in 0.15 M Tris-HCl/1 mM KH2PO4 (pH 7.4) and centrifuged at 600 ×g, and the supernatants were used to carbonyl determination as described in Section 2. Three samples per group were run, each consisting of a homogenate from a separate lot of pooled uterine horn tissue (five animals each). (Control versus EtOH-treated). bUterine horn tissue isolated from Control and EtOH-treated rats was homogenized in 0.15 M Tris-HCl/1 mM KH2PO4 (pH 7.4) and centrifuged at 600 ×g and the supernatants were used to sulfhydryl determination as described in Section 2. Three samples per group were run, each consisting of a homogenate from a separate lot of pooled uterine horn tissue (five animals each). (Control versus EtOH-treated). |