Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells

Figure 4

TIR and NaSAL decreased PQ-induced LDH release into the medium, while NAC afforded no protection. Differentiated SH-SY5Y cells were pretreated with TIR (100 μM and 1 mM), NaSAL (100 μM, 1 mM), and NAC (1 mM) 30 minutes prior to cultures exposure to PQ for 72 h. The delta increase in LDH release in units per liter (U/L) into the medium was calculated by subtraction of the respective controls (results were pooled from 3 different experiments, each experiment having 6 different culture wells per condition). The means were compared using one-way ANOVA, followed by Student-Newman-Keuls post hoc test (***𝑃<0.001 PQ versus control; ⁺⁺⁺𝑃<0.001 PQ versus PQ plus protector; ^$𝑃<0.05, ^$$𝑃<0.01 PQ plus protector low concentration versus PQ plus protector high concentration).