Figure 5: Effect of NMTMB on cell survival, NBD-PE accumulation, and mitochondrial polarization (JC-1 fluorescence) over 8 hours. Rat hepatocytes were treated with NMTMB for 30 minutes, 1 h, 2 h, 4 h, or 8 h. The data are expressed as an average of 3 (±SD) replicates from a single cell isolate. A statistically significant difference in JC-1 and NBD-PE levels between corresponding control replicates and that for NMTMB conditions was achieved by 1 h and 2 h, respectively ( , -test). As there was minimal survival observed for the higher test concentration beyond 2 h, NBDPE levels are not presented beyond 2 h.