Figure 6: Baf (30 nM) cotreatment shifted the mitochondrial polarization state of rat hepatocytes treated with NMTMB over an 8-hour time course. Rat hepatocytes were treated with NMTMB for 30 minutes, 1 h, 2 h, 4 h, 6 h or 8 h. Mitochondrial polarization status was monitored using JC-1 fluorescence on a Molecular Devices Flexstation instrument. The data are expressed as an average of 3 replicates (±SD) from a single cell isolate. Statistically significant differences between control replicates and that for all NMTMB conditions was achieved by 1 h ( , -test).