High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells
Figure 1
Expression of glycogen, albumin, alpha-1-antitrypsin, and glutathione in HepaRG and HepG2 cells seeded at 50,000 cells per well in 96-well plates and cultured for 48 hrs following thaw. (a) Periodic Acid-Schiff staining for glycogen in HepG2 (upper panels) and HepaRG (lower panels). Unstained 10x phase contrast images are shown on the left side; 10x images of cells stained with PAS reagents are shown on the right side. (b) Secretion of albumin into tissue culture medium by HepaRG and HepG2 cells. Data represent mean ± SEM for 3 independent experiments. represents versus control measurements taken from culture media which had been unexposed to cells; represents versus HepG2 cells. (c) Secretion of alpha-1-antitrypsin into tissue culture medium by HepaRG and HepG2 cells. Data represent mean ± SEM for 3 independent experiments. represents versus control measurements taken from culture media which had been unexposed to cells; represents versus HepG2 cells. (d) Levels of glutathione in untreated cells and in HepaRG and HepG2 cells treated for 12 hrs with 100 μM buthionine sulfoximine (BSO). Data represent mean ± SEM for 3 independent experiments. represents versus HepG2 cells; represents versus cells not treated with BSO.
