High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells
Figure 2
Basal and induced CYP3A4 activity in HepaRG and HepG2 cells. (a) Basal CYP3A4 activity of HepaRG and HepG2 cells plated in 96-well collagen-coated plates at seeding densities of 25,000, 50,000, and 75,000 cells per well and cultured for 72 hours. Data represent mean ± SEM for 3 independent experiments. represents versus HepG2 cells at the same seeding density; represents versus HepaRG cells seeded at 25,000 cells per well; ¤ represents versus HepaRG cells seeded at 50,000 cells per well. (b) CYP3A4 induction in HepaRG and HepG2 cells plated in 96-well plates at seeding densities of 25,000, 50,000, and 75,000 cells per well and treated with DMSO (0.1%) or rifampicin (RIF, 10 M) for 72 hrs starting at Day 3 in culture. Data represent mean ± SEM for 3 independent experiments. represents versus HepG2 cells at the same seeding density; represents versus HepaRG cells seeded at 25,000 cells per well.
