Journal of Toxicology / 2014 / Article / Fig 2

Research Article

High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells

Figure 2

Basal and induced CYP3A4 activity in HepaRG and HepG2 cells. (a) Basal CYP3A4 activity of HepaRG and HepG2 cells plated in 96-well collagen-coated plates at seeding densities of 25,000, 50,000, and 75,000 cells per well and cultured for 72 hours. Data represent mean ± SEM for 3 independent experiments. represents versus HepG2 cells at the same seeding density; represents versus HepaRG cells seeded at 25,000 cells per well; ¤  represents versus HepaRG cells seeded at 50,000 cells per well. (b) CYP3A4 induction in HepaRG and HepG2 cells plated in 96-well plates at seeding densities of 25,000, 50,000, and 75,000 cells per well and treated with DMSO (0.1%) or rifampicin (RIF, 10 M) for 72 hrs starting at Day 3 in culture. Data represent mean ± SEM for 3 independent experiments. represents versus HepG2 cells at the same seeding density; represents versus HepaRG cells seeded at 25,000 cells per well.
291054.fig.002a
(a)
291054.fig.002b
(b)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.