High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells
Multiplexed staining of cryopreserved HepaRG cells seeded at 50,000 cells per well and treated with DMSO vehicle control or 10 M rifampicin. (a) and (e): DMSO and rifampicin-treated cells, respectively, stained with Hoechst 33342 nuclear stain; (b) and (f): DMSO and rifampicin-treated cells, respectively, stained with anti-CK-19 antibody; (c) and (g): DMSO and rifampicin-treated cells, respectively, stained with anti-CYP3A4 antibody; (d) and (h): merged images of (a–c) and (e–g), respectively.
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