High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells

Figure 5

Multiplexed staining of cryopreserved HepaRG cells seeded at 50,000 cells per well and treated with DMSO vehicle control or 10 M rifampicin. (a) and (e): DMSO and rifampicin-treated cells, respectively, stained with Hoechst 33342 nuclear stain; (b) and (f): DMSO and rifampicin-treated cells, respectively, stained with anti-CK-19 antibody; (c) and (g): DMSO and rifampicin-treated cells, respectively, stained with anti-CYP3A4 antibody; (d) and (h): merged images of (a–c) and (e–g), respectively.