Table of Contents Author Guidelines Submit a Manuscript
Journal of Toxicology
Volume 2014 (2014), Article ID 291054, 12 pages
http://dx.doi.org/10.1155/2014/291054
Research Article

High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells

1Molecular and Cell Biology Systems, EMD Millipore Corporation, 290 Concord Road, Billerica, MA 01821, USA
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
3Barnes-Jewish Hospital, One Barnes-Jewish Hospital Plaza, St. Louis, MO 63110, USA

Received 11 April 2014; Revised 15 August 2014; Accepted 18 August 2014; Published 8 September 2014

Academic Editor: Cinta Porte

Copyright © 2014 Aarati R. Ranade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. Peter Guengerich, “Mechanisms of drug toxicity and relevance to pharmaceutical development,” Drug Metabolism and Pharmacokinetics, vol. 26, no. 1, pp. 3–14, 2011. View at Publisher · View at Google Scholar · View at Scopus
  2. J. R. Senior, “Drug hepatotoxicity from a regulatory perspective,” Clinics in Liver Disease, vol. 11, no. 3, pp. 507–524, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. P. McGonigle and B. Ruggeri, “Animal models of human disease: challenges in enabling translation,” Biochemical Pharmacology, vol. 87, pp. 162–171, 2014. View at Google Scholar
  4. M. J. Gómez-Lechón, M. T. Donato, J. V. Castell, and R. Jover, “Human hepatocytes as a tool for studying toxicity and drug metabolism,” Current Drug Metabolism, vol. 4, no. 4, pp. 292–312, 2003. View at Publisher · View at Google Scholar · View at Scopus
  5. J. V. Castell, R. Jover, C. P. Martínez-Jiménez, and M. J. Gómez-Lechón, “Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies,” Expert Opinion on Drug Metabolism & Toxicology, vol. 2, pp. 183–212, 2006. View at Google Scholar
  6. I. S. Behbahan, Y. Duan, A. Lam et al., “New approaches in the differentiation of human embryonic stem cells and induced pluripotent stem cells toward hepatocytes,” Stem Cell Reviews and Reports, vol. 7, no. 3, pp. 748–759, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. A. Guillouzo, A. Corlu, C. Aninat, D. Glaise, F. Morel, and C. Guguen-Guillouzo, “The human hepatoma HepaRG cells: A highly differentiated model for studies of liver metabolism and toxicity of xenobiotics,” Chemico-Biological Interactions, vol. 168, no. 1, pp. 66–73, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. P. Gripon, S. Rumin, S. Urban et al., “Infection of a human hepatoma cell line by hepatitis B virus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 24, pp. 15655–15660, 2002. View at Publisher · View at Google Scholar · View at Scopus
  9. R. Parent, M.-J. Marion, L. Furio, C. Trépo, and M.-A. Petit, “Origin and characterization of a human bipotent liver progenitor cell line,” Gastroenterology, vol. 126, no. 4, pp. 1147–1156, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Rogue, C. Lambert, C. Spire, N. Claude, and A. Guillouzo, “Interindividual variability in gene expression profiles in human hepatocytes and comparison with HepaRG cells,” Drug Metabolism and Disposition, vol. 40, no. 1, pp. 151–158, 2012. View at Publisher · View at Google Scholar · View at Scopus
  11. T. B. Andersson, K. P. Kanebratt, and J. G. Kenna, “The HepaRG cell line: a unique in vitro tool for understanding drug metabolism and toxicology in human,” Expert Opinion on Drug Metabolism & Toxicology, vol. 8, no. 7, pp. 909–920, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. C. Guguen-Guillouzo and A. Guillouzo, “General review on in vitro hepatocyte models and their applications,” Methods in Molecular Biology, vol. 640, pp. 1–40, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. C. Guguen-Guillouzo, A. Corlu, and A. Guillouzo, “Stem cell-derived hepatocytes and their use in toxicology,” Toxicology, vol. 270, no. 1, pp. 3–9, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. U. Zanelli, N. P. Caradonna, D. Hallifax, E. Turlizzi, and J. B. Houston, “Comparison of cryopreserved HepaRG cells with cryopreserved human hepatocytes for prediction of clearance for 26 drugs,” Drug Metabolism and Disposition, vol. 40, no. 1, pp. 104–110, 2012. View at Publisher · View at Google Scholar · View at Scopus
  15. M. Persson, A. F. Løye, M. Jacquet et al., “High-content analysis/screening for predictive toxicology: application to hepatotoxicity and genotoxicity,” Basic & Clinical Pharmacology & Toxicology, vol. 115, no. 1, pp. 18–23, 2014. View at Publisher · View at Google Scholar
  16. J. J. Xu, P. V. Henstock, M. C. Dunn, A. R. Smith, J. R. Chabot, and D. de Graaf, “Cellular imaging predictions of clinical drug-induced liver injury,” Toxicological Sciences, vol. 105, no. 1, pp. 97–105, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. P. J. O'Brien, W. Irwin, D. Diaz et al., “High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening,” Archives of Toxicology, vol. 80, no. 9, pp. 580–604, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. K. A. Giuliano, A. H. Gough, D. Lansing Taylor, L. A. Vernetti, and P. A. Johnston, “Early safety assessment using cellular systems biology yields insights into mechanisms of action,” Journal of Biomolecular Screening, vol. 15, no. 7, pp. 783–797, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. H. Garside, K. F. Marcoe, J. Chesnut-Speelman et al., “Evaluation of the use of imaging parameters for the detection of compound-induced hepatotoxicity in 384-well cultures of HepG2 cells and cryopreserved primary human hepatocytes,” Toxicology in Vitro, vol. 28, pp. 171–181, 2014. View at Google Scholar
  20. U. M. Zanger and M. Schwab, “Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic variation,” Pharmacology & Therapeutics, vol. 138, no. 1, pp. 103–141, 2013. View at Publisher · View at Google Scholar · View at Scopus
  21. M. S. Wilson, J. R. Graham, and A. J. Ball, “Multiparametric high content analysis for assessment of neurotoxicity in differentiated neuronal cell lines and human embryonic stem cell-derived neurons,” Neurotoxicology, vol. 42, pp. 33–48, 2014. View at Google Scholar
  22. S. Arastu-Kapur, J. L. Anderl, M. Kraus et al., “Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a link to clinical adverse events,” Clinical Cancer Research, vol. 17, no. 9, pp. 2734–2743, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. U. M. Zanger and M. Schwab, “Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic variation,” Pharmacology and Therapeutics, vol. 138, no. 1, pp. 103–141, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. H. H. J. Gerets, K. Tilmant, B. Gerin et al., “Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins,” Cell Biology and Toxicology, vol. 28, no. 2, pp. 69–87, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. T. R. van Vleet, P. J. Klein, and R. A. Coulombe Jr., “Metabolism and cytotoxicity of aflatoxin B1 in cytochrome P-450-expressing human lung cells,” Journal of Toxicology and Environmental Health A, vol. 65, no. 12, pp. 853–867, 2002. View at Publisher · View at Google Scholar · View at Scopus
  26. D. Mueller, L. Krämer, E. Hoffmann, S. Klein, and F. Noor, “3D organotypic HepaRG cultures as in vitro model for acute and repeated dose toxicity studies,” Toxicology in Vitro, vol. 28, no. 1, pp. 104–112, 2014. View at Publisher · View at Google Scholar · View at Scopus
  27. R. Jossé, C. Aninat, D. Glaise et al., “Long-term functional stability of human HepaRG hepatocytes and use for chronic toxicity and genotoxicity studies,” Drug Metabolism and Disposition, vol. 36, no. 6, pp. 1111–1118, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. S. Anthérieu, C. Chesné, R. Li et al., “Stable expression, activity, and inducibility of cytochromes P450 in differentiated HepaRG cells,” Drug Metabolism and Disposition, vol. 38, no. 3, pp. 516–525, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. J. L. Anderl, S. Redpath, and A. J. Ball, “A neuronal and astrocyte co-culture assay for high content analysis of neurotoxicity,” Journal of Visualized Experiments, no. 27, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. P. J. Lee, P. J. Hung, and L. P. Lee, “An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture,” Biotechnology and Bioengineering, vol. 97, no. 5, pp. 1340–1346, 2007. View at Publisher · View at Google Scholar · View at Scopus