Circadian Rhythms and Breast Cancer: The Role of Per2 in Doxorubicin-Induced Cell Death

Figure 5

Analysis of cell cycle progression in MDA-MB-231 breast cancer cells. MDA-MB-231 cells were subjected to (1) control conditions (C), (2) 2.5 μM doxorubicin (D), (3) 30 nM Per2 esiRNA (P), and (4) 30 nM Per2 for 48 hours + 2.5 μM Dox for 24 hours (PD). Cell cycle analysis was assessed following propidium iodide staining by flow cytometry. Statistical analysis: one way ANOVA with Bonferroni post hoc correction. All results are presented as mean ± SEM (). versus control and versus control.

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