Research Article
A Toxicological Evaluation of a Standardized Hydrogenated Extract of Curcumin (CuroWhite™)
Table 6
Summary results of the confirmatory chromosomal aberration assay (treatment without S9-mix: 18 h, harvest: 18 h).
| | Test samples | Cytotoxicity test | Aberrations/100 cells | Number of cells with aberrations | % of cells with aberrations | | Relative cell growth (RCG) | Relative mitotic index (RMI) | Chromatid type | Chromosome-type | Others | | RCG% | Cytotoxicity% | RMI% | Cytotoxicity% | Gap+ | Gap− | Gap+ | Gap− | Gap+ | Gap− | Gap− |
| | Without S9 activation | | | | | | | | | | | | | | Negative control (DMSO, 0.1%) | | | | | | | | | | | | | | Parallel Slide 1 | 100 | 0 | 100 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 2.0 | | Parallel Slide 2 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1.0 | | Mean | 1.5 | 0.5 | 0.0 | 1.0 | 0.0 | 1.0 | 1.5 | 1.5 | | Test item | | | | | | | | | | | | | | Parallel Slide 1 (5 μg/mL) | 95 | 5 | 72 | 28 | 3 | 2 | 0 | 0 | 0 | 2 | 2 | 2.0 | | Parallel Slide 2 (5 μg/mL) | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 2.0 | | Mean (5 μg/mL) | 2.0 | 1.0 | 0.0 | 1.0 | 0.0 | 1.5 | 2.0 | 2.0 | | Parallel Slide 1 (10 μg/mL) | 82 | 18 | 70 | 30 | 3 | 2 | 0 | 2 | 0 | 2 | 4 | 4.0 | | Parallel Slide 2 (10 μg/mL) | 1 | 3 | 0 | 1 | 0 | 1 | 3 | 3.0 | | Mean (10 μg/mL) | 2.0 | 2.5 | 0.0 | 1.5 | 0.0 | 1.5 | 3.5 | 3.5 | | Parallel Slide 1 (15 μg/mL) | 66 | 34 | 56 | 44 | 5 | 16 | 0 | 5 | 0 | 2 | 8 | 8.0 | | Parallel Slide 2 (15 μg/mL) | 3 | 8 | 0 | 3 | 0 | 2 | 5 | 5.0 | | Mean (15 μg/mL) | 4.0 | 12.0 | 0.0 | 4.0 | 0.0 | 2.0 | 6.5 | 6.5 | | Positive control (mitomycin C) | | | | | | | | | | | | | | Parallel Slide 1 (0.4 μg/mL) | 24 | 76 | 35 | 65 | 5 | 33 | 1 | 16 | 3 | 6 | 25 | 25.0 | | Parallel Slide 2 (0.4 μg/mL) | 3 | 49 | 0 | 4 | 0 | 3 | 37 | 37.0 | | Mean (0.4 μg/mL) | 4.0 | 41.0 | 0.5 | 10.0 | 1.5 | 4.5 | 31.0 | 31.0 |
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DMSO: dimethylsulfoxide. Notes. Gap+ indicates scores including gaps. Gap− indicates scores excluding gaps. “Others” indicates polyploid cells and endoreduplicated metaphases. Statistical analysis was conducted on % aberrant cells using the chi-square test.
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