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Journal of Toxicology
Volume 2018, Article ID 6849765, 8 pages
Research Article

In Vivo and In Vitro Toxicity Evaluation of Hydroethanolic Extract of Kalanchoe brasiliensis (Crassulaceae) Leaves

1Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, RN, Brazil
2Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, RN, Brazil
3Department of Pharmacy, Federal University of Pernambuco, Recife, PE, Brazil
4Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil

Correspondence should be addressed to Aldilane Gonçalves Fonseca; moc.liamtoh@acesnofenalidla and Telma Maria Araújo Moura Lemos; rb.moc.oohay@lmamlet

Received 24 October 2017; Accepted 19 December 2017; Published 21 January 2018

Academic Editor: Shakir Ali

Copyright © 2018 Aldilane Gonçalves Fonseca et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The species Kalanchoe brasiliensis, known as “Saião, has anti-inflammatory, antimicrobial, and antihistamine activities. It also has the quercetin and kaempferol flavonoids, which exert their therapeutic activities. With extensive popular use besides the defined therapeutical properties, the study of possible side effects is indispensable. The objective of this study is to evaluate the toxicity in vitro and in vivo from the hydroethanolic extract of the leaves of K. brasiliensis. The action of the extract (concentrations from 0.1 to 1000 uL/100 uL) in normal and tumor cells was evaluated using the MTT method. Acute toxicity and subchronic toxicity were evaluated in mice with doses of 250 to 1000 mg/kg orally, following recognized protocols. The in vitro results indicated cytotoxic activity for 3T3 cell line (normal) and 786-0 (kidney carcinoma), showing the activity to be concentration-dependent, reaching 92.23% cell inhibition. In vivo, the extract showed no significant toxicity; only liver changes related to acute toxicity and some signs of liver damage, combining biochemical and histological data. In general, the extract showed low or no toxicity, introducing itself as safe for use with promising therapeutic potential.