Table 1: Summary table of the results of the initial mutation test.

Initial mutation test (plate incorporation test)

Concentrations (g/plate)Salmonella typhimurium tester strainsEscherichia coli
TA 98TA 100TA 1535TA 1537WP2uvrA
-S9+S9-S9+S9-S9+S9-S9+S9-S9+S9

Mean values of revertants per plate and mutation rate (MR)MeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMR

Untreated control13.70.8919.31.0984.01.02105.01.128.71.1811.71.0312.70.9311.30.8913.30.9521.71.16
DMSO control15.31.0017.71.0082.01.0093.71.007.31.0011.31.0013.71.0012.71.0014.01.0018.71.00
Ultrapure Water control83.31.008.31.0014.01.00

500011.00.7214.00.7954.00.6678.00.833.70.504.70.414.70.342.00.1613.00.9318.00.96
160013.30.8718.01.0256.00.6870.30.759.31.278.00.7112.70.939.70.7610.70.7618.00.96
50012.30.8022.01.2560.70.7486.30.928.71.1810.00.8810.00.7312.00.9511.30.8119.31.04
16011.00.7223.01.3089.31.0992.30.998.01.0911.31.0011.70.8512.71.0014.31.0219.71.05
5015.31.0024.01.3698.31.2087.00.937.31.009.70.8512.00.8813.71.0811.00.7916.70.89
1615.31.0020.01.1386.01.0591.70.989.01.239.00.7912.00.8812.71.0014.01.0017.70.95
513.30.8717.00.9684.31.03101.01.089.01.2312.01.0613.30.9812.30.9714.01.0018.30.98

NPD (4)208.013.57
SAZ (2)1008.012.101066.7128.00
9AA (50)574.042.00
MMS (2 μL)422.730.19
2AA (2)1469.383.172506.726.76105.09.26136.710.79
2AA (50)209.011.20

DMSO was used as the vehicle for the test article and positive control substances: NPD, 9AA, and 2AA. Ultrapure water was used as the vehicle for SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO.