Mutagenicity and Acute Oral Toxicity Test for Herbal Poultry Feed Supplements

Table 5

KOLIN PLUS, mutagenicity test results.

Strain	S9	Untreated control	Vehicle control (DMSO)	KOLIN PLUS					Positive control
				39.0 μg/ml	78.1 μg/ml	156.2 μg/ml	312.5 μg/ml	625 μg/ml
S. typhimurium
TA 98		0.67 ± 1.15	2.00 ± 0.00	1.67 ± 1.15	0.33 ± 0.58	0.00 ± 0.00	0.00 ± 0.00	1.33 ± 0.58	48.00 ± 0.00
		0.67 ± 1.15	1.00 ± 1.00	1.33 ± 0.58	1.33 ± 0.58	3.00 ± 1.73	2.67 ± 2.51	0.67 ± 1.15	35.67 ± 2.52
TA 100		2.00 ± 2.65	1.67 ± 0.58	2.33 ± 0.58	2.00 ± 2.65	1.00 ± 1.73	1.33 ± 1.15	1.67 ± 2.08	45.33 ± 1.53
		5.33 ± 1.15	3.33 ± 0.58	4.00 ± 1.00	3.33 ± 1.53	2.33 ± 0.58	3.33 ± 1.53	2.00 ± 1.73	48.00 ± 0.00
TA 1535		2.00 ± 1.00	0.67 ± 1.15	0.67 ± 0.58	1.33 ± 2.31	0.67 ± 0.58	1.00 ± 1.00	1.33 ± 0.58	48.00 ± 0.00
		0.67 ± 0.58	0.33 ± 0.58	1.00 ± 0.00	3.00 ± 4.36	1.33 ± 1.53	2.00 ± 2.00	2.33 ± 2.52	42.67 ± 2.08
TA 1537		0.00 ± 0.00	0.00 ± 0.00	1.33 ± 0.58	0.33 ± 0.58	0.00 ± 0.00	0.67 ± 0.58	0.67 ± 0.58	40.67 ± 0.58
		1.00 ± 1.00	0.33 ± 0.58	1.00 ± 1.00	0.33 ± 0.58	0.33 ± 0.58	1.67 ± 1.53	0.67 ± 0.58	41.67 ± 2.08
E. coli
WP2 uvrA		0.67 ± 0.58	1.00 ± 1.00	1.33 ± 0.58	1.00 ± 1.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	43.00 ± 3.61
		0.67 ± 0.58	1.00 ± 1.00	1.67 ± 1.53	0.33 ± 0.58	0.33 ± 0.58	1.00 ± 1.00	0.67 ± 0.58	46.00 ± 2.65

Revertant colonies (mean ± SD). Statistically significant (); positive controls. With S9: 2-aminoanthracene (i) 5 μg/ml, TA 98, TA 100, TA 1535, and TA 1537; (ii) 50 μg/ml, E. coli WP2 uvrA; without S9: 2-nitrofluorene—2 μg/ml, TA 98; 4-nitroquinoline-N-oxide—0.1 μg/ml, TA 100; N⁴-aminocytidine—100 μg/ml, TA 1535; 9-amino-1,2,3,4-tetra hydro acridine hydrochloride—15 μg/ml, TA 1537; 4-nitroquinoline-N-oxide—1.0 μg/ml, E. coli WP2 uvrA.