Journal of Toxicology / 2019 / Article / Tab 2

Research Article

A Toxicological Evaluation of Methylliberine (Dynamine®)

Table 2

Results of the confirmatory mutation test.

Salmonella typhimurium tester strainsEscherichia coli
Concentrations (µg/plate)TA 98TA 100TA 1535TA 1537WP2uvrA
−S9+S9−S9+S9−S9+S9−S9+S9−S9+S9
Mean number of revertants per plate mutation rateMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMR
Untreated control16.71.3922.71.3364.71.0596.71.3010.71.079.00.778.71.188.01.0929.71.0935.30.89
DMSO control12.01.0017.01.0061.71.0074.31.0010.01.0011.71.007.31.007.31.0027.31.0039.71.00
Ultrapure water control71.31.007.71.0029.71.00
Test item
500016.01.3317.01.0026.30.4324.70.338.70.877.70.6610.71.458.71.1831.71.1634.00.86
160018.71.5619.71.1650.00.8169.30.939.30.937.70.6612.01.647.71.0533.71.2333.30.84
50014.71.2220.71.2261.30.9988.71.1911.71.179.00.778.01.097.00.9525.70.9439.00.98
16018.31.5324.71.4563.01.0298.01.3210.71.0711.00.948.31.147.71.0525.30.9342.31.07
5019.01.5824.71.4562.71.0299.01.3310.01.0010.30.897.71.058.01.0920.00.7340.71.03
1615.71.3119.01.1262.01.0194.01.268.00.8011.30.976.00.827.71.0527.00.9940.01.01
510.30.8618.71.1058.70.9596.31.3011.31.1311.30.976.70.918.31.1428.31.0441.01.03
Positive controls
NPD (4 μg/plate)490.040.83
SAZ (2 μg/plate)1354.718.99850.7110.96
9AA (50 μg/plate)333.045.41
MMS (2 μL/plate)897.330.25
2AA (2 μg/plate)1213.371.37866.711.66118.310.1482.711.27
2AA (50 μg/plate)164.34.14

Abbreviations: MR, mutation rate; DMSO, dimethyl sulfoxide; NPD, 4-Nitro-1,2-phenylenediamine; SAZ, sodium azide; 9AA, 9-aminoacridine; MMS, methyl methanesulfonate; 2AA, 2-aminoanthracene. Remarks: DMSO was applied as vehicle of the test item and the positive control substances: NPD, 9AA, and 2AA. Ultrapure water was applied as vehicle for the positive control substances: SAZ and MMS. The mutation rates of the test item, untreated control, NPD, 9AA, and 2AA are given referring to DMSO. The mutation rates of the SAZ and MMS positive controls are given referring to the ultrapure water.

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