Toxicological Evaluation of a Mixture of Astragalus membranaceus and Panax notoginseng Root Extracts (InnoSlim®)

Table 2

Summary of the results of the confirmatory mutation test.

Confirmatory Mutation Test (Pre-Incubation Test)
Concentrations (μg/plate)	Salmonella typhimurium tester strains																Escherichia coli
	TA 98				TA 100				TA 1535				TA 1537				WP2uvrA
	-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9
Mean values of revertants per plate Mutation rate (MR)	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR
Untreated Control	12.7	0.97	17.7	0.70	69.3	1.10	91.0	0.93	11.0	0.79	11.3	0.87	6.7	0.83	8.7	0.93	26.7	1.01	37.3	1.24
DMSO Control	14.7	1.00	25.3	1.00	–	–	85.3	1.00	–	–	12.0	1.00	6.0	1.00	9.3	1.00	–	–	35.3	1.00
Ultrapure Water Control	13.0	1.00	25.3	1.00	63.0	1.00	97.7	1.00	14.0	1.00	13.0	1.00	8.0	1.00	9.3	1.00	26.3	1.00	30.0	1.00
TEST ITEM
5000	17.3	1.33	24.7	0.97	83.0	1.32	96.0	0.98	17.7	1.26	12.7	0.97	8.3	1.04	7.7	0.82	36.0	1.37	36.7	1.22
1600	15.3	1.18	23.3	0.92	78.0	1.24	98.7	1.01	12.7	0.90	12.3	0.95	8.7	1.08	9.0	0.96	28.3	1.08	35.7	1.19
500	18.0	1.38	20.3	0.80	71.3	1.13	94.3	0.97	13.0	0.93	13.0	1.00	6.3	0.79	9.7	1.04	39.7	1.51	37.3	1.24
160	12.0	0.92	23.7	0.93	85.3	1.35	104.3	1.07	12.0	0.86	10.0	0.77	9.3	1.17	9.3	1.00	29.7	1.13	40.0	1.33
50	11.0	0.85	19.0	0.75	76.7	1.22	99.3	1.02	11.3	0.81	13.7	1.05	6.7	0.83	8.0	0.86	31.7	1.20	31.0	1.03
16	17.0	1.31	21.3	0.84	69.3	1.10	104.3	1.07	11.0	0.79	13.3	1.03	8.7	1.08	9.3	1.00	30.3	1.15	40.0	1.33
POSITIVE CONTROLS
NPD (4 μg/plate)	460.7	31.41		–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
SAZ (2 μg/plate)	–	–	–	–	625.3	9.93	–	–	1157.3	82.67	–	–	–	–	–	–	–	–	–	–
9AA (50 μg/plate)	–	–	–	–	–	–	–	–	–	–	–	–	364.7	60.78	–	–	–	–	–	–
MMS (2 μL/plate)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	1258.7	47.80	–	–
2AA (2 μg/plate)	–	–	677.3	26.74	–	–	726.7	8.52	–	–	107.0	8.92	–	–	145.7	15.61	–	–	–	–
2AA (50 μg/plate)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	156.3	4.42

MR, mutation rate; DMSO, dimethyl sulfoxide; NPD, 4-nitro-1,2-phenylenediamine; SAZ, sodium azide; 9AA, 9-aminoacridine; MMS, methyl methanesulfonate; 2AA, 2-aminoanthracene
Remarks. Ultrapure water was applied as vehicle of the test item and the positive control substances: SAZ and MMS; and the DMSO was applied as vehicle for positive control substances: NPD, 9AA, and 2AA. The mutation rate of the test item, SAZ, MMS, and untreated control refers to the ultrapure water; the mutation rate of NPD, 9AA, and 2AA refers to DMSO.