Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study

Figure 6

Detection by immunocytochemistry of 4-hydroxynonenal (4-HNE) in liver of controls (a, e) and in rats exposed toTiO₂ nanoparticles (16g/kg) and sacrificed after 4 days (b-d; f-h). Oxidative stress induced a production of 4-HNE in some hepatocytes of treated rats (b) as compared to controls (a). In treated animals (c, d), hepatocytes showing an oxidative stress (arrows) were exclusively located around centrilobular veins (CV). No immunoreactivity was detected around portal triad (PT) of control rats (e) by contrast to treated animals showing some deeply stained cells (arrows) in this area (f). At high magnification, (g, h) these 4-NHE positive cells (arrows) appeared as Kupffer cells principally localized in Mall’s space around bile ducts (B). PV: branches of portal vein; A: branches of hepatic artery.

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