Comparison of In Vitro Endocrine Activity of Phthalates and Alternative Plasticizers

Figure 4

E-Screen antagonist competitive confirmation assay for DINP and DEHT. MCF-7 cells were exposed for 120 h to DEHT 300 μM or DINP 30 μM in presence of increasing concentrations of E2. ICI 182,780 (ICI) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were used as antiestrogenic and cytotoxic reference compounds. Results represent the mean ± SD of 3 replicates.