Comparison of In Vitro Endocrine Activity of Phthalates and Alternative Plasticizers

Figure 8

Estradiol production in H295R cells exposed to phthalates and substitutes. H295R cells were exposed for 48 h to different concentrations of each phthalate and substitute, solvent control (SC), and forskolin 10 μM (F) and prochloraz 1 μM (P) as reference inducer and inhibitor of steroidogenesis. (c) Cytotoxic concentration (viability < 80%) is not analyzed. Results represent the mean fold change in hormone concentration compared to SC (=1) ±SD of 3 replicates from one representative assay out of 2 assays. ^∗p < 0.05, . (a) DEHP. (b) DINP. (c) DEHT. (d) DINCH. (e) TOTM. (f) POLYSORB® isosorbide. (g) POLYSORB® ID 46.

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