Cytotoxicity of Particulate Matter PM₁₀ Samples from Ouagadougou, Burkina Faso

Figure 2

Production of ROS in HPAEC cells after 4 hours of exposure to PM₁₀ of districts 2 (a), 3 (b), and 4 (c) of Ouagadougou (Burkina Faso). The HPAEC cells were seeded for 24 hours and then treated with different concentrations of PM₁₀ (1.5, 10, 25, and 50 µg/cm²) for 4 hours. Intracellular ROS production was evaluated by the CM-H2DCFDA probe and measured by spectrofluorimetric method. The percentage increase in ROS is calculated relative to control cells. Data are expressed as means ±SEM of three independent experiments n = 3 (three wells per concentration). The statistical test used is a one-way ANOVA followed by a multiple comparison with Dunnett’s correction, : significance level compared to control cells.

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